| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083325 |
| Chromosome: | chromosome 7 |
| Location: | 5965290 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354550 | FAP65 | (1 of 18) 3.5.1.98 - Histone deacetylase / HDAC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGATGGTGCGGCGCGCCCTCCAGTAA |
| Internal bar code: | GAGCCAGACAACGGTAACAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 12.3435 |
| LEAP-Seq n confirming: | 286 |
| LEAP-Seq n nonconfirming: | 2031 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACTGTAAAAACACCCGT |
| Suggested primer 2: | TCAAACAGCTGAAGAAGGCA |