| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083339 |
| Chromosome: | chromosome 3 |
| Location: | 7278041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207200 | (1 of 18) PF00650 - CRAL/TRIO domain (CRAL_TRIO) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGCGGCGGCCCTCGCCGCGCGCGCAG |
| Internal bar code: | GGCCTCCGATTACTCTAGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCAGAGTTCAGCGGGTC |
| Suggested primer 2: | GGGCTGAAGGCGTATGATTA |