Insertion junction: LMJ.RY0402.083351_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.083351
Chromosome:chromosome 13
Location:4605550
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g604050 (1 of 1) IPR002048//IPR006626//IPR011050 - EF-hand domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCGAGGCCGCAGAGAGCTCCCCGAAGG
Internal bar code:TCTCCAACATTGATCGTGTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:340
LEAP-Seq percent confirming:68.5988
LEAP-Seq n confirming:1503
LEAP-Seq n nonconfirming:688
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAAGGAGTTGGGGAGTTG
Suggested primer 2:ATCTCTTCCTCCGTCACCCT