Insertion junction: LMJ.RY0402.083351_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.083351
Chromosome:chromosome_13
Location:4605550
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g604050 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGCCGAGGCCGCAGAGAGCTCCCCGAAGG
Internal bar code:TCTCCAACATTGATCGTGTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:340
LEAP-Seq percent confirming:68.5988
LEAP-Seq n confirming:1503
LEAP-Seq n nonconfirming:688
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAAGGAGTTGGGGAGTTG
Suggested primer 2:ATCTCTTCCTCCGTCACCCT