Insertion junction: LMJ.RY0402.083351_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.083351
Chromosome:chromosome_13
Location:4605582
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g604050 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GGATTACGGTGTTGCCTTTAACGACTATGA
Internal bar code:TGCTTGGACTGAGACGGCTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:2.05371
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:1240
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAAGGAGTTGGGGAGTTG
Suggested primer 2:ATCTCTTCCTCCGTCACCCT