| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083354 |
| Chromosome: | chromosome 6 |
| Location: | 3128674 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGCCGGCTAAGGGCGCAGCGTGAAGG |
| Internal bar code: | TCGGTAGGCCGATACCCTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 99.1379 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATTCGCTCATGATTAGA |
| Suggested primer 2: | CACCTCAAATGGTAACCGCT |