| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083384 |
| Chromosome: | chromosome 6 |
| Location: | 4881775 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279000 | PBA1 | (1 of 2) K02738 - 20S proteasome subunit beta 1 (PSMB6); 20S proteasome beta subunit A1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGATGTACGAGCGGGAGGCCACTTGCGC |
| Internal bar code: | TTGTTCAAGCTAAAAGGCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 98.9083 |
| LEAP-Seq n confirming: | 2265 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGTGTTGCGTTGGTGAA |
| Suggested primer 2: | CTAGCCTGCCCATACAGAGC |