| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083389 |
| Chromosome: | chromosome 2 |
| Location: | 5640961 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACATGAGTTGGTTGGCGGACCAAGCGA |
| Internal bar code: | TATTCTCCCTTTTCCCGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.6422 |
| LEAP-Seq n confirming: | 12531 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGCCGTACTACATGCG |
| Suggested primer 2: | TCGTCTATTGTGGGTGTGGA |