| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083400 |
| Chromosome: | chromosome 11 |
| Location: | 3275580 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480050 | EXN14 | Exoribonuclease with BTB/POZ domain; (1 of 8) PTHR26379:SF144 - BTB/POZ DOMAIN-CONTAINING PROTEIN 19 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAGCCACCATGCAGTGCGCGATTCTTC |
| Internal bar code: | GGGGTCTTCTGCAAGGAGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 98.0372 |
| LEAP-Seq n confirming: | 949 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGTTTGCGTGTTAGGA |
| Suggested primer 2: | GCACTGATGGTGAGGTCAGA |