| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083416 |
| Chromosome: | chromosome 6 |
| Location: | 1267786 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258150 | (1 of 1) PTHR14659:SF1 - ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATGGCACCGCACAGTGGCATATAGAAA |
| Internal bar code: | GAGTAGGGTTGCCTCATCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4 |
| LEAP-Seq percent confirming: | 99.8086 |
| LEAP-Seq n confirming: | 2608 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTGAGGTGAGTTGGGGT |
| Suggested primer 2: | TCGGTTTCCATCCTCGATAG |