| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083419 |
| Chromosome: | scaffold 18 |
| Location: | 106636 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748797 | (1 of 1) PTHR32026:SF10 - PROTEIN Y39F10A.3-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTCGAAAGAGGGGCGGAACGGATTCGT |
| Internal bar code: | GGCGGGAAGCTTGGCGCGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 98.9911 |
| LEAP-Seq n confirming: | 4906 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGGAAGGAAGTAGGGC |
| Suggested primer 2: | GCAGTCGGGCTCAGATGTAT |