Insertion junction: LMJ.RY0402.083447_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.083447
Chromosome:chromosome 6
Location:4000110
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278199 (1 of 4) 1.13.11.75 - All-trans-8'-apo-beta-carotenal 15,15'-oxygenase / 8'-apo-beta-carotenal 15,15'-oxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGGGTACGTCTGCCTGGAGCCGCGCTT
Internal bar code:ATGCCCGCGCGGGTAGCTGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:83.1551
LEAP-Seq n confirming:311
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACAGGGAGGTGTGTGTGTG
Suggested primer 2:CACACATTCAACAGTTCGGG