| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083469 |
| Chromosome: | chromosome 14 |
| Location: | 1240985 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616400 | (1 of 8) PF00011 - Hsp20/alpha crystallin family (HSP20) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCACGATGAGCACCCCGTCGCGCAGCG |
| Internal bar code: | CTGTTGAACGGTTCCAGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 22.2703 |
| LEAP-Seq n confirming: | 669 |
| LEAP-Seq n nonconfirming: | 2335 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTGAACACCATTGCATC |
| Suggested primer 2: | CCCCATTTGCTGACTACGTT |