| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083513 |
| Chromosome: | chromosome 11 |
| Location: | 3610042 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482001 | FAP208 | Ankyrin Repeat Flagellar Associated Protein 208; (1 of 1) K13118 - protein DGCR14 (DGCR14) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGACATACAACCCCACCCCCACAAAGC |
| Internal bar code: | ATTGGGGCGACGCTGACTAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 98.9157 |
| LEAP-Seq n confirming: | 821 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTTACATTCGTGATTGG |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |