Insertion junction: LMJ.RY0402.083527_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.083527
Chromosome:chromosome 10
Location:5686865
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g460600 CYN53 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 53 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCCTGCGCAGCAACCAGTCGCCCGTGG
Internal bar code:AATCGCTGGGGTATCGTTATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:49.9218
LEAP-Seq n confirming:319
LEAP-Seq n nonconfirming:320
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCAACCATATGCAACAGT
Suggested primer 2:CTTGAAGTGGTAGGGCTTGC