| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083546 |
| Chromosome: | chromosome 3 |
| Location: | 2408876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159016 | (1 of 13) 2.7.11.1//2.7.11.25 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Mitogen-activated protein kinase kinase kinase / MLTK | 5'UTR_intron|intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCATCAACGGGCCAACAACGGGGCAA |
| Internal bar code: | CTACCGTGGTACTTTAGATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1230 |
| LEAP-Seq percent confirming: | 99.0308 |
| LEAP-Seq n confirming: | 7663 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTCTCTGACCCTTGCGCT |
| Suggested primer 2: | GGAAGACCGCAGACAGAGAC |