| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083546 |
| Chromosome: | chromosome_3 |
| Location: | 2409073 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g159016 | antisense | 5'UTR|CDS|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTCGCTCACATTCTGCGAAGCAACAAAGG |
| Internal bar code: | GTTCACTCGGCAGGATATAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 96.4072 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGAAGCGAGCTTACAGCA |
| Suggested primer 2: | GGAAGACCGCAGACAGAGAC |