| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083567 |
| Chromosome: | chromosome 16 |
| Location: | 1136133 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650151 | SPAST1 | (1 of 2) PF05496//PF09336 - Holliday junction DNA helicase ruvB N-terminus (RuvB_N) // Vps4 C terminal oligomerisation domain (Vps4_C); Spastin homologue | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCTCCCATGCCCCTTTGGCACATGCAC |
| Internal bar code: | GCTAGTAAGTCGTTGCAAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 97.9343 |
| LEAP-Seq n confirming: | 1849 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGGTAGTGGCGAAGTGG |
| Suggested primer 2: | GCATCACCCCTTGTATGCTT |