| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083571 |
| Chromosome: | chromosome 14 |
| Location: | 2404167 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g624450 | (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCCCGCTTCCTCTTATGCTCACACTC |
| Internal bar code: | ACGCCCAGTTAGTCGCATGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5 |
| LEAP-Seq percent confirming: | 99.7579 |
| LEAP-Seq n confirming: | 5356 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGGCGTGTAACGAGTA |
| Suggested primer 2: | GACACCACCATGCTCATCAC |