Insertion junction: LMJ.RY0402.083616_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.083616
Chromosome:chromosome 11
Location:2884579
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g477850 TXC1,TSN1 (1 of 1) K15979 - staphylococcal nuclease domain-containing protein 1 (SND1); Transcriptional coactivator-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTCAGGGCGCAGACAGTAGGGGGACGGG
Internal bar code:TGTGACCTGATCATGGCGCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:212
LEAP-Seq percent confirming:98.9462
LEAP-Seq n confirming:1784
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGTGTACACAAACAGGG
Suggested primer 2:TCTACGGTACTCACGACCCC