| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083619 |
| Chromosome: | chromosome 2 |
| Location: | 4002360 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096950 | (1 of 1) PF09444 - MRC1-like domain (MRC1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACAGACCACATGGCGTTCGGAGGCGCGC |
| Internal bar code: | TCGGCATTCGCCCGTTATCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 1520 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTCCAGTTCGTCCTCAA |
| Suggested primer 2: | GGTTCAGGACACACCGAACT |