Insertion junction: LMJ.RY0402.083638_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.083638
Chromosome:chromosome 2
Location:6726997
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g118200 (1 of 3) PF01940 - Integral membrane protein DUF92 (DUF92) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCCGGTGTTGAGCAATGACGCGGTGAA
Internal bar code:CGAGTGCAAATCGGTATGACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:374
LEAP-Seq percent confirming:99.2704
LEAP-Seq n confirming:2449
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGGCTTGAGCTCCTGTTC
Suggested primer 2:TCTCTCCTCGCTTTCTCTGC