| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083640 |
| Chromosome: | chromosome 5 |
| Location: | 756284 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248550 | (1 of 1) K16276 - zinc finger protein-like protein (K16276, BTS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAATTTCCCCCTCGTAGCTCGCGCCCGT |
| Internal bar code: | TCTAAAACGTGATTAGGTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCATTGACCACATCTTC |
| Suggested primer 2: | GAACAGCTGCTCCTCCTGAC |