| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083644 |
| Chromosome: | chromosome 7 |
| Location: | 4558328 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343900 | (1 of 2) IPR011038 - Calycin-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACCGCCGTACCCAACCACCGCACCCAA |
| Internal bar code: | CTCATAGGGCCGTGACGGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 51.0713 |
| LEAP-Seq n confirming: | 1311 |
| LEAP-Seq n nonconfirming: | 1256 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTGTATGTGGTGGGGT |
| Suggested primer 2: | CACACATTCACTCCCGACAC |