| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083644 |
| Chromosome: | chromosome 10 |
| Location: | 5444482 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458500 | ASK1,AHD2 | Aspartate kinase; (1 of 1) K00928 - aspartate kinase (lysC) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCTTCTGAGAAGCAATTTGGTCAAGTA |
| Internal bar code: | GCATAGGGACTTTAGGATGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 98.972 |
| LEAP-Seq n confirming: | 1733 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATTTGAAGTGCGCGAGTA |
| Suggested primer 2: | CTGCGTGGTGTAACTAGCCA |