| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083647 |
| Chromosome: | chromosome 12 |
| Location: | 8124206 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551350 | LAO2 | (1 of 1) 3.5.99.5 - 2-aminomuconate deaminase; Periplasmic L-amino acid oxidase, accessory subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACCGTCCCCGCCACGCCCCACGCCCGT |
| Internal bar code: | AGCCGGACGGGCGTCCCGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 98.7986 |
| LEAP-Seq n confirming: | 1727 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCTCCTGTCTCACCCG |
| Suggested primer 2: | TGTATGCTTGCAGATCCGAG |