Insertion junction: LMJ.RY0402.083676_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.083676
Chromosome:chromosome 2
Location:3274717
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACAATGGTGCCAGTAGCAGTAGCAGCA
Internal bar code:GGGGAGAATTGGGAGCGGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:72
LEAP-Seq percent confirming:74.7423
LEAP-Seq n confirming:145
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGAGGAGGAGTTTGAGGG
Suggested primer 2:GAGCACGCGTTCTAAACACA