| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083696 |
| Chromosome: | chromosome 6 |
| Location: | 2100291 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265150 | (1 of 1) K11833 - ubiquitin carboxyl-terminal hydrolase 2/21 [EC:3.4.19.12] (USP2_21) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTGCCGCGCCCTAGCAGCCGCACCTCA |
| Internal bar code: | GCTGACTTACGATGTGGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 90.9465 |
| LEAP-Seq n confirming: | 442 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCAAACATTGAACACC |
| Suggested primer 2: | CAGCGTGTACTGCTGTTGGT |