| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083698 |
| Chromosome: | chromosome 8 |
| Location: | 3882280 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377950 | (1 of 3) PF02622 - Uncharacterized ACR, COG1678 (DUF179) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGTTGCGGAGAGTGTCGCTATCACAGG |
| Internal bar code: | AGTTTCTACACATTCTCATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1123 |
| LEAP-Seq percent confirming: | 99.8331 |
| LEAP-Seq n confirming: | 598 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCCTCCTTTTGTCTCG |
| Suggested primer 2: | TTGCAGTGCATACGGATGTT |