Insertion junction: LMJ.RY0402.083717_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.083717
Chromosome:chromosome 1
Location:982081
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g005500 (1 of 2) PTHR10783//PTHR10783:SF3 - XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED // VACUOLAR TRANSPORTER CHAPERONE 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGTAAAGAGGGCCCGAGCCTAGTTGACG
Internal bar code:CCCCATAAACTCGTCAGCCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:368
LEAP-Seq percent confirming:99.1608
LEAP-Seq n confirming:709
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TATCGCTTGTGTGAGCTTGG
Suggested primer 2:CCGTGAACGTCTTGAACAGA