| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083725 |
| Chromosome: | chromosome 16 |
| Location: | 6267539 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674850 | (1 of 1) PTHR14625//PTHR14625:SF3 - MICROCEPHALIN // MICROCEPHALIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCCGCGGTGGCCGCAAGGCCTCCGCTG |
| Internal bar code: | GGTAAATCGCAGTGCATGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 53.5484 |
| LEAP-Seq n confirming: | 332 |
| LEAP-Seq n nonconfirming: | 288 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCTTCAGCAGAGGAAGT |
| Suggested primer 2: | ACAGTAGTAGGTGGTGCCGC |