| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083726 |
| Chromosome: | chromosome 10 |
| Location: | 6486722 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466400 | (1 of 8) IPR011257 - DNA glycosylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAACACATCTTCTGAGTGCGGAGCTCTG |
| Internal bar code: | GGGAACGTCGCGTTCATCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 98.3027 |
| LEAP-Seq n confirming: | 4170 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTTGGGACTGCACTGTT |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |