| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083735 |
| Chromosome: | chromosome 2 |
| Location: | 3207756 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095071 | (1 of 2) K01148 - poly(A)-specific ribonuclease (PARN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTCTTTTAGCACTGAGTGCACACGTGT |
| Internal bar code: | CGGTTCGCAGCCCACGTCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 44.6608 |
| LEAP-Seq n confirming: | 711 |
| LEAP-Seq n nonconfirming: | 881 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAACTAGTGGAGTGTGCG |
| Suggested primer 2: | GTATGAAGTGCCGTGAGGGT |