Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.083746 |
Chromosome: | chromosome 6 |
Location: | 1952782 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g263800 | SEE2 | Putative splicing endonuclease positive effector; (1 of 1) PTHR10887:SF369 - DNA2/NAM7 HELICASE FAMILY PROTEIN | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGCTGCCGTCCCCCACAAAACCGTCAT |
Internal bar code: | CGTCCCCGGACCGCGCGAGTAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 82 |
LEAP-Seq percent confirming: | 97.1831 |
LEAP-Seq n confirming: | 69 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCATTACCATGTGCCCCT |
Suggested primer 2: | CTTGCTTCACTGTCATGCGT |