| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083757 |
| Chromosome: | chromosome 3 |
| Location: | 8085912 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201050 | BSD2,ZNJ2 | (1 of 10) 5.3.4.1 - Protein disulfide-isomerase / S-S rearrangase; Bundle-sheath defective-2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACTGCGCCGGTGCACAGCCGCGACACT |
| Internal bar code: | GGATGGATTAACGTCTCGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.2042 |
| LEAP-Seq n confirming: | 1122 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGCTTTTCTTGGTGCCT |
| Suggested primer 2: | GCTTAACTTCCCCCACCTTC |