| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083789 |
| Chromosome: | chromosome_3 |
| Location: | 8086498 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g201000 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATCCTGCCAAAAGCCTCGCTAAGCGTTGA |
| Internal bar code: | ACGGCCATCAGGTGGCGGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 35.8718 |
| LEAP-Seq n confirming: | 3090 |
| LEAP-Seq n nonconfirming: | 5524 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGAGGTAGGCTCGAAG |
| Suggested primer 2: | GCTACGGCAACTTTGGAGAG |