Insertion junction: LMJ.RY0402.083799_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.083799
Chromosome:chromosome 12
Location:600259
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g492500 (1 of 39) IPR009003 - Peptidase S1, PA clan intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCTGCAACCCCGCCTGCGTGCCACCTGC
Internal bar code:ACTGTCACCTAGAACACTATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1472
LEAP-Seq percent confirming:99.613
LEAP-Seq n confirming:44790
LEAP-Seq n nonconfirming:174
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:AGTTCAACAAGCACATCCCC
Suggested primer 2:CTAGGTTCTTCGCCATGCTC