| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.083803 |
| Chromosome: | chromosome 8 |
| Location: | 1203134 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363150 | (1 of 2) PTHR23301//PTHR23301:SF0 - CHITIN BINDING PERITROPHIN-A // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGAAAATGGTGGCATGCCTCTTCGCTC |
| Internal bar code: | CTACGTAGGCTGTTCCGGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGCGTTGTGCACCTCTA |
| Suggested primer 2: | AACCTGTGTAGGGTCAACGC |