| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083831 |
| Chromosome: | chromosome 5 |
| Location: | 2554014 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236039 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGGGGGTGAGGGACGGGGCGGTATGTA |
| Internal bar code: | GTGCAATGGTGATGTTCTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 98.2802 |
| LEAP-Seq n confirming: | 3486 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCATAGCCCAGAAGATG |
| Suggested primer 2: | GCTAAGCAAGCACCACAACA |