| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083860 |
| Chromosome: | chromosome 10 |
| Location: | 682673 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422350 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTGGGGCGCTATCTGGCAGGTAGAGC |
| Internal bar code: | AATTTATAATTCGAACTTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 98.8199 |
| LEAP-Seq n confirming: | 1591 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTACAGGCATCGTCCGAG |
| Suggested primer 2: | AGTAAGCCCTCCCTAGCTGC |