| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.083860 |
| Chromosome: | chromosome 10 |
| Location: | 682762 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422350 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCGCTATCAACGGAAGCACTACTCATT |
| Internal bar code: | CAATATAGTGGATTATCTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 20.0428 |
| LEAP-Seq n confirming: | 281 |
| LEAP-Seq n nonconfirming: | 1121 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTACAGGCATCGTCCGAG |
| Suggested primer 2: | AGTAAGCCCTCCCTAGCTGC |