Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.083871 |
Chromosome: | chromosome_9 |
Location: | 2586027 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g390550 | sense | 5'UTR_intron|intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCTGGTGCAGGTGGGCGGGCGGGGGGTGG |
Internal bar code: | CCAATCGACTTGGAGCAGCGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 83 |
LEAP-Seq percent confirming: | 66.6667 |
LEAP-Seq n confirming: | 6 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCCCTTTGCACTGCTCTAC |
Suggested primer 2: | CCTGCTTCCTCACCTCTGAC |