Insertion junction: LMJ.RY0402.083885_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.083885
Chromosome:chromosome 9
Location:3667630
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g390578 TRP23,FAP11 (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL; Flagellar Associated Protein 11 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTGCTTCTGTCACTTCGAATAAGACAAT
Internal bar code:AGGCATGTGCGGTTGCAATGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:660
LEAP-Seq percent confirming:98.6686
LEAP-Seq n confirming:1334
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCCATCAAGACGCTTTC
Suggested primer 2:GACGGCTGTGAAGCAGTACA