| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083897 |
| Chromosome: | chromosome_13 |
| Location: | 2070751 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g577352 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTCCAAAACTTTGTTTCGGTTGCTGCAAG |
| Internal bar code: | GCGGAGCGTGGATGCCGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 98.5436 |
| LEAP-Seq n confirming: | 5007 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATGACCGCTTCTGTTGGG |
| Suggested primer 2: | GTAGTGGCCAATGTCTCGGT |