| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.083904 |
| Chromosome: | chromosome 1 |
| Location: | 2171957 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011850 | (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCACTTTGTCCGACTCGTTCGTCGTGA |
| Internal bar code: | TCCTTACATTGGCGGCCTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 95.1537 |
| LEAP-Seq n confirming: | 805 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGCTTTCATTCCGTG |
| Suggested primer 2: | AACATAAAATGGCACGCTCC |