| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083917 |
| Chromosome: | chromosome 5 |
| Location: | 419645 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232750 | CNK4 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF311 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCCCGAATCGACCTCCCTAGACCCGAC |
| Internal bar code: | ACTGGCAGCTTGCCACATCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.3925 |
| LEAP-Seq n confirming: | 818 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCATCACCTGAAATGGT |
| Suggested primer 2: | CATCTGGATGACCTCGGACT |