Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.083920 |
Chromosome: | chromosome_16 |
Location: | 1627707 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g654300 | RSP23 | Radial spoke protein 23 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGGCGGCAGAGGCGGCGACACTTGTGCAG |
Internal bar code: | CAGCCCACCGTGCCAACTGTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1057 |
LEAP-Seq percent confirming: | 98.2517 |
LEAP-Seq n confirming: | 562 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCCTCAGCTCTACAACGTC |
Suggested primer 2: | AGTTCGTGTATCCCGACAGC |