| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.083978 |
| Chromosome: | chromosome 17 |
| Location: | 1901856 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710200 | IAD1 | Indoleamine 2%252C3-dioxygenase; (1 of 1) 1.13.11.11 - Tryptophan 2,3-dioxygenase / Tryptophanase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGCCGCGCGTAAGCATGGGCCGGAGGG |
| Internal bar code: | ACGAAGGACGGTGTGTGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 98.1539 |
| LEAP-Seq n confirming: | 90704 |
| LEAP-Seq n nonconfirming: | 1706 |
| LEAP-Seq n unique pos: | 255 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACACAATTCACAACGAC |
| Suggested primer 2: | GCTGAATCCAGAAGGAGCAG |