| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084025 |
| Chromosome: | chromosome 6 |
| Location: | 2419489 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268450 | (1 of 1) PTHR22850//PTHR22850:SF85 - WD40 REPEAT FAMILY // WD-40 REPEAT-CONTAINING PROTEIN MSI4-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTCACGCACAGGCGCGGAGCAGGGCAG |
| Internal bar code: | CTACGCAAGTGATAGTTAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.6893 |
| LEAP-Seq n confirming: | 5454 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCTTGTCCGTCTTCTTC |
| Suggested primer 2: | CTGTGCCTGGCTACACTTCA |