| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.084028 |
| Chromosome: | chromosome 9 |
| Location: | 4577030 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396326 | (1 of 1) PF02213 - GYF domain (GYF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGCACGGAGTTGATGTGTTGCCGGGG |
| Internal bar code: | ATATGTTCAGGGCAGTTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 98.9324 |
| LEAP-Seq n confirming: | 1390 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCCTCCTCCCTAACT |
| Suggested primer 2: | GTCGAAGAGCACGAGGAAAC |