Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.084038 |
Chromosome: | chromosome 6 |
Location: | 1051583 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g256420 | (1 of 1) K15196 - transcription factor IIIB 90 kDa subunit (BRF1, GTF3B) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCCCGGGAGCCACAATGCCAATACAGG |
Internal bar code: | ACGGCGCCTTGCATCTCGGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 32 |
LEAP-Seq percent confirming: | 86.2903 |
LEAP-Seq n confirming: | 107 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGATCAACTACGGAGCCC |
Suggested primer 2: | CGGACAGGAATGAAGCAAAT |