| Insertion cassette: | CIB1 | 
| Side of cassette: | 5' | 
| Strand: | - | 
| Strain: | LMJ.RY0402.084038 | 
| Chromosome: | chromosome 6 | 
| Location: | 1051583 | 
| Confidence (%): | 58 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre06.g256420 | (1 of 1) K15196 - transcription factor IIIB 90 kDa subunit (BRF1, GTF3B) | 3'UTR | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCCCGGGAGCCACAATGCCAATACAGG | 
| Internal bar code: | ACGGCGCCTTGCATCTCGGCGG | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 | 
| LEAP-Seq percent confirming: | 86.2903 | 
| LEAP-Seq n confirming: | 107 | 
| LEAP-Seq n nonconfirming: | 17 | 
| LEAP-Seq n unique pos: | 2 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGATCAACTACGGAGCCC | 
| Suggested primer 2: | CGGACAGGAATGAAGCAAAT |